Volume 56 (2006) Issue: 2006 No#5-6

Examination of the activity of glycoprotein HN and F antigens of the outer envelope of the parainfluenza virus type 3 by using fusional, hemolytic and hemagglutinating tests, in vitro

Author(s): Nišavić J, Milić N

Keywords:virus PI3, fusional test, hemagglutination, hemolysis

The objective of our study was to examine fusional, hemolytic and hemagglutinating activities of the surface glycoprotein HN and F antigens of parainfluenza virus type 3, in vitro. The samples of activated PI3 virions, induced Vero cell fusion after 24 h and 48 h. After treatment of inoculated Vero cells was the dilutions of specific immune sera against PI3 virus, cell fusion were not registered at dilutions of 1:2, 1:4 and 1:8 of the immune sera. The samples of PI3 virions, activated by 0.025 g/dL trypsin-versen, induced hemolysis at antigen dilution of 1:8. The samples of activated PI3 virions, expressed an intensive hemagglutinating activity of 128 HAU/0.1 mL. After treatment of the abovementioned samples with specific immune sera against PI3 virus, hemolytic activities were not detected at the dilution of 1:16 of specific immune sera. These results showed that the fusional and hemolytic tests can be used for fast detection of immunologically important glycoprotein antigens of PI3 virus and their identification with specific immune sera.