Volume 55 (2005) Issue: 2005 No#2-3

Salmonella enteritidis isolation from broiler chickens infected with low doses

Author(s): Maja Velhner, Stojanov I, Potkonjak Dubravka, Kapetanov M, Orlić D, Rašić Z

Keywords:Salmonella enteritidis, broiler chickens, experimental infection, bacteriology, serology

In this paper we present the possibilities for detection of Salmonella enteritidis (SE) by bacteriological and serological technics. Also, we studied which tissue is the most suitable sample for Salmonella isolation. One day old and three weeks old broiler chickens were experimentally infected with low doses of SE. For the isolation of SE we used Rappaport Vassiliadis media. Specific antibodies in sera were detected using ELISA. According to our results, broiler chickens were susceptible to infection with low doses of SE. However, specific antibodies could not been found. SE was isolated from caeca, but not from livers of infected chickens when tested at the end of the experiment (at 6 weeks). Our results revealed that bacterial cultures are still the method of choice for detection of SE in broilers flocks.