Volume 55 (2005) Issue: 2005 No#5-6

Characterization of transcription factors binding to -120 GATA motif of rat
b miny – globin promoter

Author(s): Pavlović Sonja, Mitrović Tatjana, Karan-Đurašević Teodora, Nikčević Gordana

Keywords:rat
-globin genes, transcription regulation, GATA-1

The aim of this study was to elucidate the regulation of rat adult
b miny-globin gene transcription. We used DNaseI footprinting, gel mobility shift and supershift assays to characterize transcription factors involved in this regulation. In this study we analyzed GATA motif at – 120 bp in the distal promoter of
b miny-globin gene. Footprint analysis revealed the binding of nuclear factors from MEL cells to the GATA motif. By using gel mobility shift assay two protein complexes were detected. The faster migrating complex was erythroid-specific and more abundant in differentiating MEL cells. Competition experiments with GATA-1 oligonucleotides and GATA-1 protein antibodies confirmed binding of GATA-1 transcription factor to GATA motif at – 120 bp regulation of rat adult
b miny-globin gene.