Volume 55 (2005) Issue: 2005 No#5-6

Study of dentate gyrus granule cells of female rats neonatally treated with sex hormones

Author(s): Drekić D, Mrvić Verica, Lozanče Olivera, Kerkez M, Blagojević M, Zorić Z

Keywords:testosterone dipropionate, estrogen dipropionate, dentate gyrus, neurons, female rat

This study was designed to determine whether adult neurogenesis occurs in the dentate gyrus of rats, and to explore the possibility that this process is regulated by the activation of sex hormones. The proliferation of hippocampal dentate gyrus granule cells was investigated using 80
Ci 3H - thymidine incorporation in the first group andin the second grouph 80
Ci 3H - thymidine and 1mg estrogene dipropionae. We proved the existence of a stronger proliferation of granule cells in SGZ GD in female rats. The third group of newborn female Wistar rats was treated at 4 and 8 a days of ageold, with a single dose of 2 mg of testosterone propionate. We proved the existence of minor damages of granule cells in SGZ GD. In the fourth group, treatedat 15 days of age,a single dose of 4 mg testosterone propionate and sacrificed when 60 days old, we proved the existence of minor damages of granular cells in SGZ GD. Our results suggest a differential effect of sex hormones on dentate gyrus granule cells proliferation through early life in rats.