Volume 54 (2004) Issue: 2004 No#4

A novel dot blot test for Trichinella spiralis antigen detection

Author(s): Ilić Nataša, Petrović M, Djordjevic M, Sofronić-Milosavljević Ljiljana

Keywords:dot blot, ES antigen, polyclonal antibodies, Trichinella spiralis

The Trichinella spiralis excretory-secretory (ES) antigen complex contains components characteristic for the whole genus. Besides their strong immunomodulatory effect, they are key targets for the host immune system. These antigens are currently used for research purposes and also in serological diagnostics. Immunodiagnostic tools, based on ES antigen application, provide an indirect confirmation of parasitism, ensure specific antibody detection and accurate diagnosis in infected humans and are recommended for surveillance programs in animals. If recognized in antibody fluids of the host, the ES antigen is direct evidence of infection. In our study, polyclonal anti-ES antibodies were produced, characterized and analyzed for application in a dot blot test. Applied as capture antibodies, they were successfully combined with monoclonal antibody 7C2C5 which recognizes a Trichinella genus specific epitope. The lowest detectable antigen concentration, i.e. dot blot test sensitivity was 156 ng/ml. This dot blot test may be considered specific and sufficiently sensitive. Validated in an experimental model system, it is expected to have a possible application in practice in Serbia with the ongoing reemergence of trichinellosis.