Volume 54 (2004) Issue: 2004 No#5-6

Distribution of CB1 cannabinoid receptors in the rat amygdaloid complex

Author(s): Puškaš L, Puškaš Nela, Todorović Vera, Malobabić S, Labudović-Borović Milica, Đulejić V

Keywords:immunohistochemistry, CB1 receptors, amygdaloid nuclei, rat

The amygdaloid complex (AK) has a very important role in the modulation of endocrine and visceral functions, in complex behavioral mechanisms such as defence, feeding, aggression, affects, reproduction, memory and learning. The aim of this study was to determine the precise distribution of cannabinoid CB1 receptors in the rat AK, using the immunohistochemical (ABC) method. According to our results, CB1-immunoreactivity in the rat AK was highest in the medial nucleus. Slightly lower immunoreactivity was found in the basolateral nucleus. Moderate density of CB1 receptors occurred in the central, basomedial, lateral and posterior nuclei of the AK. CB1-immunoreactivity in all of these nuclei was present in the form of discrete spot-like precipitates of unequal size and appearance. These precipitates exhibited three different patterns: 1. elongated columns or lines, 2. complete or incomplete rings and 3. in a small number of AK regions CB1-immunoreactivity was separately dispersed in the form of single spot-like precipitates between more complex columns and rings of precipitates. Considering the functional importance of amygdala and the distribution of CB1 receptors in the AK we could conclude that our findings suggest a role for cannabinoids in modulating responses of the AK to stress and fear as well as to pain.