Volume 53 (2003) Issue: 2003 No#2-3

AN EXPERIMENTAL INTRAOPERATIVE QUANTITATIVE ECHOCARDIOGRAPHIC STUDY OF MYOCARDIAL PERFUSION IN DOGS

Author(s): Aranđelović Aleksandra, Pavlović S, Milašinović G, Mrdović I, Ašanin Milika, Žunič Snežana, Malobabić S

Keywords:echocardiography, dogs, coronary occlusion, myocardial perfusion, contrast medium

Myocardial contrast echocardiography is a valuable technique for demonstration and delineation of regions of myocardial underperfusion secondary to coronary occlusion and/or significant coronary stenosis. Various contrast materials have been used. The aim of this study was to determine whether myocardial contrast echocardiography by the original contrast agent AQ-DDT (albumin based), produced in the Institute for Cardiovascular Diseases, Clinical Center of Serbia in Belgrade can be used in the detection and quantification of regions of myocardial perfusion defects. In 14 adult open chest dogs the perfusion defect was produced by selective coronary ligations (30 seconds) and was observed with epicardial contrast two-dimensional echocardiography. For administration of contrast a modified pigtail catheter was positioned in the aorta just above the aortic valves. In this way the perfusion regions of the left main and right coronary artery were included. The left anterior descending and left circumflex artery could not be selectively injected with contrast agent and their individual perfusion areas were not clearly demonstrated. In all cases contrast echocardiography images of the left ventricle were obtained in a short-axis cross-sectional view at the mid-papillary muscle level. Forty-two injections of AQ-DDT for perfusion analysis were done, and were recorded on a VHS recorder. Quantification of the quality in demonstrating myocardial perfusion was scored as good, poor or without visual echocardiographic effect, by an investigator experienced in echocardiography, immediately and one month later. There was 100% agreement in the scoring. Our results indicated that agent AQ-DDT produces a good contrast effect (echocardiography visualization) in dogs and has the potential to demonstrate regional perfusion defects of the myocardium. Its potential role in human medicine, for diagnosis and evaluation of the results of interventional therapy, especially during aortocoronary bypass graft surgery, should be evaluated.