Volume 53 (2003) Issue: 2003 No#4

TOLUENE MEDIATED OXIDATIVE STRESS AND GRANULO-MONOCYTOPOIESIS

Author(s): Božić Tatjana, Stevanović Jelka, Kovačević Milica, Jović S, Lukić Sanja, Petakov Marijana, Borozan Sunčica, Mijačević Zora, Knežević Milijana, Bulajić Snežana

Keywords:rat, ceruloplasmin, hematological indices, malondialdehyde, propylene glycol, toluene

The influence of toluene exposure on some biochemical and hematological parameters was investigated in adult female Wistar rats. The animals were subjected to intraperitoneal administration of toluene diluted in propylene glycol and the diluent alone for 3, 7 and 11 consecutive days at the same time intervals. The effects of toluene and propylene glycol were evaluated biochemically by determining the plasma concentrations of total proteins, albumin and ceruloplasmin (Cp) together with erythrocyte malondialdehyde (MDA), and hematological indices from peripheral blood and bone marrow. The biochemical acute phase response was manifested by an elevated Cp concentration in all experimental animals. The markedly enhanced MDA concentration and statistically significant decrease in albumin level in toluene treated rats, indicated damage, to blood vessel endothelia. Alterations of leukocytes of peripheral blood and bone marrow (BM) granulocytic-monocytic progenitor cells were typical of an inflammatory response, with stimulation of granulo-monocytopoiesis. Therefore, it can be assumed that both toluene and propylene glycol mediated sterile peritonitis and oxidative stress injury, with changes intensified by toluene action.