Volume 53 (2003) Issue: 2003 No#5-6

EXAMINATION OF ANTIGENIC STRUCTURE AND SOME BIOLOGICAL ACTIVITIES OF HEMAGGLUTININ-NEURAMINIDASE (HN) AND FUSION (F) GLYCOPROTEIN ANTIGENS OF PARAINFLUENZA 3 VIRUS, IN VITRO

Author(s): Milić N, Gađanski-Omerović Gordana, Ašanin Ružica, Nišavić J, Radojičić Marina

Keywords:PI3 virus, glycoprotein antigens, SDS-PAGE, BT cells, fusional test, hemolysis

The objective of our study was examination of the antigenic structure, fusional and hemolytic activities of the surface glycoprotein HN and F antigens of purified parainfluenza (PI 3) viruses activated with 0.025 g/dl trypsin-versen (molecular weights of 112 kD, 81-82 kD and 30-31 kD), in vitro. The samples of activated PI3 virions with total protein concentrations of 0.55 and 0.27mg/ml and hemagglutinating titre of 256 and 128 HAU/0.1 ml, induced bovine turbinate(BT) cell fusion and hemolysis of guinea-pig erythrocytes. After treatment of the aforementioned samples with specific hyperimmune sera against PI 3 virus in which the hemagglutination-inhibition (HI) titre was 1:64, cell fusion was not registered. The results show that there are possibilities to use fusional and hemolytic tests for the fast detection of imunologically important glycoprotein antigens of PI 3 viruses and their identification with specific hyperimmune sera.