Volume 62 (2012) Issue: 2012 No#2-3

Regional distribution of opiate alkaloids in experimental animals' brain tissue and blood

Author(s): Đurendić-Brenesel Maja, Pilija V, Cvjetićanin S, Ivetić Vesna, Mimica-Dukić Neda

Keywords:brain tissue, experimental animals, GC-MS, heroin, opiates' distribution

The aim of this study was to examine the regional distribution of opiate alkaloids from seized heroin in experimental animals' brain regions and blood. Results could be used in the examination of opiate alkaloids' distribution in human biological samples in order to contribute to the solution of the causes of death due to heroin intake. Experimental animals (Wistar rats) were treated with seized heroin, and were sacrificed at different time periods: 5, 15, 45 and 120 min after treatment. Opiate alkaloids' (codeine, morphine, acetylcodeine, 6- acetylmorphine and 3,6-diacetylmorphine) content was determined in the brain regions (cortex, brainstem, amygdala and basal ganglia) and blood of animals using gas chromatography–mass spectrometry (GC–MS) method. The highest content of opiate alkaloids in the blood was measured 15 min, and in the brain tissue 45 min after the treatment with heroin. The maximal concentration of opiates was determined in the basal ganglia. The obtained results offer the possibility of selecting this part of the brain tissue as a representative sample for identifying and assessing the content of opiates.