Volume 60 (2010) Issue: 2010 No#2-3

PHENOTYPE CHANGES INDUCED BY IMMUNIZATION WITH ENCEPHALITOGEN AFFECTED THE FUNCTIONS OF PERITONEAL MACROPHAGES IN TWO RAT STRAINS WITH DIFFERENT SENSITIVITY TO EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS (EAE) INDUCTION

Author(s): Mitić Katarina, Miletić Tatjana, Kovačević-Jovanović Vesna, Kuštrimović Nataša, Kosec D, Dimitrijević Mirjana, Stanojević Stanislava

Keywords:experimental autoimmune encephalomyelitis (EAE), hydrogen peroxide (H2O2), nitric oxide (NO), phagocytosis, peritoneal cells phenotype, rat strains

We have investigated the phenotype of peritoneal cells and the functions of peritoneal macrophages obtained from experimental autoimmune encephalomyelitis (EAE)-susceptible Dark Agouti (DA) and EAE-resistant Albino Oxford (AO) rat strains on days 1, 3 and 7 post immunization with encephalitogen. Resident peritoneal cells from immunized and non-immunized rats of both strains were subjected to flow cytometric analyses and after adherence were tested for zymosan phagocytosis, hydrogen peroxide (H2O2) and nitric oxide (NO) production. In non-immunized rats, macrophages from the DA rat strain phagocytosed more zymosan but produced less H2O2 than cells from the AO strain, while both strains produced comparable amounts of NO. Immunization increased phagocytosis in DA rats' cells, but decreased both phagocytosis and H2O2 production in cells from AO rats. Overall higher phagocyte ability in DA rats was associated with a significantly larger population of ED1+ cells (macrophages and dendritic cells), in contrast to a more pronounced expression of ED2 antigen (resident macrophages) on cells from AO rats. Immunization also increased the expression of CD11b molecule on non-resident ED2– macrophages of DA, but not of AO rats. The early and subtle phenotype changes in peritoneal cells of both rat strains might mirror the mechanism contributing to their different sensitivity to the induction of autoimmunity.