Volume 60 (2010) Issue: 2010 No#5-6

Cytogenetic changes in human lymphocytes induced by Alphachlorhydrine

Author(s): Teodorović Radislava, Stanimirović Z, Radenković-Damnjanović Brana, Vučinić Marijana, Janković Ljiljana, Đorđević M, Mirilović M

Keywords:Alphachlorhydrine, cytogenetic, genotoxic effects, hyman, lymphocytes

This paper deals with the results of the investigation carried out with regard to genotoxic properties of Alphachlorhydrine administered at doses of 7.5, 15 and 30 mg/kg of cell culture-human lymphocytes, during an exposition time of three days, under in vitro conditions. The assessment of the genotoxic effects of the tested chemosterilants was performed on the basis of numerical and structural aberrations in three experimental groups, in addition to one control group for each dose. On the basis of the obtained results it can be conculded that Alphachlorhydrine, administered in the abovementioned doses, induced genotoxic effects on human lymphocyte cells. The number of numeric aberrations increased with a dose increase, and the statistical analysis showed significant differences (p<0.01) in relation to the control group, but not between the applied doses (p<0.05). Analysis of the obtained results demonstarted that a dose increase during the same exposition time, resulted in a statistically significant increase (p<0.01) of structural aberrations in human lymphocyte cells; the highest level being achieved with the highest administered dose (30 mg/kg c.c.). As for Robertsonian translocations, there was a statistical significance with relation to the control group only in the case of the highest administered dose (30 mg/kg c.c.). Statistically significant differences (p<0.01) were recorded between the 30 mg/kg c.c. and 7.5 mg/kg c.c. doses. Also there were statistically significant differences between the levels of gaps and fragments in comparison to Robertsonian translocations (p<0.01); while the numbers of gaps and fragments were not significantly different.