Volume 57 (2007) Issue: 2007 No#1

Examination of the activity of HN and F glycoprotein antigens of the outer envelope of newcastle disease virus by using fusional, hemolytic, hemagglutination and hemadsorption tests, in vitro

Author(s): Nišavić J, Milić N, Veljović LJ

Keywords:Newcastle disease virus, fusion test, hemagglutination, hemolysis, hemadsorption

The objective of our study was to examine fusional, hemolytic, hemagglutination and hemadsorption activities of the surface glycoprotein HN and F antigens of Newcastle disease (ND) virus, in vitro. The samples of activated ND virions, induced Vero cell fusion after 6h, 12h, 24h and 48h. After 24h of treatment of the inoculated Vero cells with dilutions of the specific immune sera against ND virus, cell fusion was not registered at dilutions of 1:2 and 1:4. The ND virion samples, activated with 0.025 g/dL trypsin-versen, induced hemolysis of chicken erythrocytes at antigen dilutions of 1:4 and 1:8. The samples of activated ND virions, expressed an intensive hemagglutinating activity of 256 HAU/0,1 mL. After treatment of the abovementioned samples with specific immune sera against ND virus, hemolytic activities were not detected at immune sera dilution of 1:32. The hemadsorption of chicken erythrocytes at the surface of inoculated Vero cells was detected after 6h of inoculation with activated ND viruses. After 24h of treatment of inoculated Vero cells with dilutions of specific immune sera against ND virus, the hemadsorption of chicken erythrocytes was not registered at immune sera dilution of 1:64. These results showed the possibility to use fusion, hemolytic and hemadsorption tests for the detection of immunologically important glycoprotein antigens of ND viruses and their identification with specific immune sera.