Volume 57 (2007) Issue: 2007 No#1

Changes in enzymes activity superoxide dismutase and glutathione reductase, reduced glutathione and quantity of malonil dialdehyde after acute aluminum poisoning

Author(s): Milovanović J, Milovanović A, Konstantinović Ljubica, Đorđević V, Stanković P, Babić B, Arsović N, Đukić V

Keywords:aluminum poissoning, enzymes activity, superoxiddismutase, glutathione reduktase, lipid peroxidation

The important and increasing application of aluminum motivated us to determine whether and in what way does the antioxidative status of erythrocytes in aluminum acute poisoned animals change by studying the metabolism of superoxid dismutase (SOD), glutathione reductase (GR), reduced glutathione (GSH) and malonil dialdehyde (MDA). The experiment involved desert mice (gerbils) who received intraperitonally an aluminum chloride solution at a dose of 3.7 g/kg BM. The experimental groups of animals were sacrified after 24, 48, 72 and 96 hours from the beginning of the experiment by cardial punction or by punction of the abdominal artery. To the control group saline solution was applied. It has been noticed that during the course of the experiment SOD activity increased. We assume this represents a defense mechanism of the organism to increased quantities of free radicals. Further, aluminum considerably reduces the glutathione reductive system capacity affecting its enzymatic component, glutathione reductase which leads to a further decrease in reduced glutathione. The lipid peroxidation index expressed through MDA as an indicator of membrane status remains unchanged. This could mean that malonil dialdehyde is measured in erythrocytes not jet structurally damaged.