Volume 65 (2015) Issue: 2015 No#2

Multiplication of the enterocyte mass by serosal patch technique

Author(s): Grebeldinger Slobodan, Radojčić Branka, Ćulafić Jelena, Andrejić Višnjić Bojana

Keywords:bioengineering, colony-stimulating factors, enterocytes, small intestine, patch technique.

Damage to the small intestine and impairment of the intestinal epithelium occur in various diseases, resulting in a need for new epithelium. Therefore, bioengineering of the small intestine is becoming an attractive field of research where all contributions are highly appreciated. The purpose of this study was to determine the possibility of the multiplication of the enterocyte mass using the technique of serosal patch with the application of hematopoietic stem cells, as well as the assessment of the quality of newly formed mucosa. Sixty Mill Hill hooded rats were divided in 4 groups, 15 animals each. In the control group animals, the patch was not created. In the other three groups, the animals were operated on and in each group 8 parietal and 7 visceral patches have been created. One of the groups with operated animals (Group NS) was not postoperatively treated. The second group of operated animals (Group G) was stimulated with granulocyte colony-stimulating factor (G-CSF). The third group of operated animals (Group GM) was stimulated with recombined humane granulocyte-macrophage colony-stimulating factor (rHuGM-CSF). In the group of animals that were not stimulated, epithelium proliferated slowly. In the group of animals stimulated with G-CSF stimulants, the epithelium initially proliferated rapidly, but appeared atrophic after eight weeks. Stimulation by rHuGM-CSF led to faster epithelization, and epithelium showed signs of advancing proliferation after eight weeks. We confirmed the possibility of enterocyte mass multiplication by using the serosal patch technique, as well as that stimulation with rHuGM-CSF is more effective than stimulation with G-CSF.