Volume 55 (2005) Issue: 2005 No#2-3

Porcine stress syndrome (PSS) and ryanodine receptor 1 (RYR 1) gene mutation in european wild pig (Sus scrofa ferus)

Author(s): Jovanović S, Trailović Ružica, Savić Mila, Sarač M

Keywords:wild pigs, PSS, halothane test, RYR1 locus

Expression of the stress syndrome (PSS) in European wild pig was evaluated by Halothane test and tested animals were typed by PCR/RFLP test for C/T mutation at nt 1843 in RYR1 gene. A total of 56 animals, at the age of 5 months, weighing 20 kg average, were tested. Hal+ (MHS – malignant hyperthermia susceptible) phenotype was revealed in 6 pigs, 47 animals expressed Hal– (MHN – malignant hyperthermia resistant) phenotype, while 3 animals showed no response to administration of 3% halothane after 5 minutes and they were marked as Halr (halothane resistant) phenotypes. Genotyping of 56 wild pigs revealed that the tested population was homozygous for the normal C/C allele at RYR1 locus. The results obtained by halothane test show that expression of the PSS in wild pigs is modulated and influenced by factors other then RYR1 genotype.